fhuB [2018-02-10T14:20:29.000Z]

hydroxamate siderophore ABC transporter (ferrichrome und ferrioxamine) (permease)

Locus: BSU33310

Isoelectric point: 10.14

Molecular weight: 40.56 kDa

Protein length: 384 aa

Gene length: 1152 bp

Function: siderophore uptake

Product: hydroxamate siderophore ABC transporter (ferrichrome und ferrioxamine) (permease)

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Importers → Uptake of iron/ siderophores
• Cellular processes → Homeostasis → Acquisition of iron → ABC transporters for the uptake of iron/ siderophores
• Metabolism → Additional metabolic pathways → Iron metabolism → ABC transporters for the uptake of iron/ siderophores
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,417,222 → 3,418,376

The protein

Protein family

• FecCD subfamily (according to Swiss-Prot)

Paralogous protein(s)

• YfiZ, FecD

Structure

• 4G1U (from Yersinia pestis, 37% identity) PubMed

Localization

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: fhuB-fhuG-fhuC

  Description

  • PubMed

  Regulatory mechanism

  • Fur: repression, PubMed, in Fur regulon

  Regulation

  • induced by iron starvation (second wave to allow iron scavenging from the environment) (Fur) PubMed

Biological materials

Mutant

• BKE33310 (ΔfhuB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTGCAAAAATATCGTGT, downstream forward: _UP4_CGTCGTGAAAGGAGAGGGCT
• BKK33310 (ΔfhuB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTGCAAAAATATCGTGT, downstream forward: _UP4_CGTCGTGAAAGGAGAGGGCT

References

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